[ Methodology & handling ]
Aliquot
A measured portion of a working stock, often frozen separately to avoid repeated freeze-thaw cycles.
To aliquot is to divide a reconstituted working stock into smaller measured portions. Researchers commonly aliquot and freeze a stock so that each portion is thawed once, rather than repeatedly freezing and thawing a single vial.
Repeated freeze-thaw cycles can degrade a peptide, so aliquoting is a handling step that protects sample integrity across a longer research programme.
[ Where this term is used ]
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